Protein expression of the α, γ, δ and ε subspecies of protein kinase C changes as C6 glioma cells become contact inhibited and quiescent in the presence of serum

FEBS Letters

Moreton, Kevin²  Paton, Alex¹  Rumsby, Martin²  Turner, Rebecca²  Groome, Nigel¹  Blake, Nicola² 

[1]School of Molecular Sciences, Oxford Brookes University, Oxford, OX3 0BP, England, UK

[2]Department of Biology, University of York, York, YO1 5YW, England, UK

关键词: Contact inhibition;    C6 glioma cell;    Protein kinase C subspecies expression changes;    Quiescence;    G0;    ATP;    adenosine-5-triphosphate;    BCA;    biocinchoninic acid;    CB;    column buffer;    DAG;    diacylglycerol;    DMEM;    Dulbecco's modified Eagle's medium;    ECL;    enhanced chemiluminescence;    EDTA;    ethylenediaminetetraacetic acid;    EGTA;    ethylene glycol-bis(β-aminoethyl ether)-N;    N;    N′;    N′-tetraacetic acid;    FBS;    foetal bovine serum;    HB;    homogenisation buffer;    MBP;    myelin basic protein;    PBS;    phosphate-buffered saline;    PKC;    protein kinase C;    PMSF;    phenylmethyl sulphonyl fluoride;    SDS;    sodium dodecylsulphate;    TBS;    Tris-buffered saline;    TBST;    Tris-buffered saline/0.2% Tween 20;   

DOI  :  10.1016/0014-5793(95)00941-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Total protein kinase C (PKC) activity and protein expression of the α and δ subspecies of PKC increases markedly as C6 glioma cells grow from low cell density to the contact-inhibited quiescent state (also known as G₀) in the presence of serum. At the same time protein expression of PKC subspecies γ and ϵ decreases while the β _I, β _II, ι and ζ subspecies did not change. Serum deprivation of growing C6 glioma cells does not induce the same changes in PKC subspecies protein expression. The findings support the growing view that there are significant differences between the G₀ states brought about by contact inhibition or serum deprivation.

【 授权许可】

Unknown