Isolation, characterization, and chromosomal location of a gene encoding the Δ 1‐pyrroline‐5‐carboxylate synthetase in Arabidopsis thaliana

FEBS Letters

Savouré, Arnould¹  Ardiles, Wilson¹  Verbruggen, Nathalie¹  Van Montagu, Marc¹  Hua, Xue-Jun¹  Jaoua, Samir¹ 

[1] Laboratorium voor Genetica, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Arabidopsis thaliana;    Pyrroline-5-carboxylate synthetase;    Proline;    Salt stress;    P5C;    pyrroline-5-carboxylate;    PCR;    polymerase chain reaction;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(95)00935-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA and the corresponding At-P5S gene encoding the first enzyme of the proline biosynthetic pathway, the Δ ¹-pyrroline-5-carboxylate (P5C) synthetase, were isolated in Arabidopsis thaliana. The At-P5S cDNA encodes a protein of 717 amino acids showing high identity with the P5C synthetase of Vigna aconitifolia. Strong homology is also found at the N-terminus to bacterial and yeast γ-glutamyl kinase and at the C-terminus to bacterial γ-glutamyl phosphate reductase. Putative ATP- and NAD(P)H-binding sites are suggested in the At-P5S protein. The transcribed region of the At-P5S gene is 4.8 kb long and contains 20 exons. Southern analysis suggests the presence of only one At-P5S gene in the A. thaliana genome mapped at the bottom of the chromosome two. Expression analysis of At-P5S in different organs reveals abundant At-P5S transcripts in mature flowering plant. Rapid induction of the At-P5S gene followed by accumulation of proline was observed in NaCl-treated seedlings suggesting that At-P5S is osmoregulated.

【 授权许可】

Unknown