Identification and characterization of two isoforms of an endothelin‐converting enzyme‐1

FEBS Letters

Tanzawa, Kazuhiko²  Takahashi, Masaaki²  Shimada, Kohei²  Ikeda, Masahiro¹ 

[1] Pharmacology and Molecular Biology Research Laboratories, Sankyo Co. Ltd., 1-2-58 Hiromachi, Shinagawa-ku, Tokyo 140, Japan;Biological Research Laboratories, Sankyo Co. Ltd., 1-2-58 Hiromachi, Shinagawa-ku, Tokyo 140, Japan

关键词: Endothelin-converting enzyme;    Endothelin;    Alternative splicing;    Rat ECE-1β;   

DOI  :  10.1016/0014-5793(95)00886-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cloning and sequencing of 5′-terminal region of a β form of rat ECE-1 cDNA which is different only in its N-terminal amino-acid sequence to the cDNA we have cloned previously (α form [K. Shimada et al. (1994) J. Biol. Chem. 269, 18275–18278]). No significant difference was found in the specific activity and substrate specificity between the two isoforms. The expression level of ECE-1α mRNA was higher than that of ECE-1β in various rat cells and tissues, suggesting that the physiologically important isoform is ECE-1α. The present findings verified the presence of two forms of ECE-1 over many species, which are created probably through alternative splicing.

【 授权许可】

Unknown