FEBS Letters | |
The derivation of the oxygen atoms of the 131‐oxo and 3‐acetyl groups of bacteriochlorophyll a from water in Rhodobacter sphaeroides cells adapting from respiratory to photosynthetic conditions: evidence for an anaerobic pathway for the formation of isocyclic ring E | |
Schäfer, W.2  Katheder, Ingrid1  Scheer, H.1  Porra, R.J.1  | |
[1] Botanisches Institut der Universität München, D-80638 München, Germany;Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany | |
关键词: Biosynthesis; Bacteriochlorophyll a; Isocyclic ring E; Anaerobic cyclization; 18O-labelling; Mass spectrometry; BChl; bacteriochlorophyll; BPhe; bacteriopheophytin; Chl; chlorophyll; Chlide; chlorophyllide; The IUPAC-IUB numbering (see Fig. 1) and nomenclature systems [19] have been used together with [] bracket notation for substituents; | |
DOI : 10.1016/0014-5793(95)00854-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Using mass spectrometry, we have demonstrated 18O-labelling of both the 131-oxo and 3-acetyl groups of newly-formed bacteriochlorophyll a synthesized by Rhodobacter sphaeroides cells during adaptation from respiratory to photosynthetic conditions in the presence of H2 18O. This derivation of the 131-oxo group of bacteriochlorophyll a from water provides a stark constrast with that of chlorophylls in higher plants where ring E formation is an aerobic process in which the 131-oxo group arises from molecular oxygen via an oxygenase activity. The formation of the 3-acetyl group of bacteriochlorophyll a, however, is consistent with the enzymic hydration of the 3-vinyl group of a derivative of chlorophyll a.
【 授权许可】
Unknown
【 预 览 】
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