FEBS Letters | |
Thrombopoietin (TPO) induces tyrosine phosphorylation and activation of STAT5 and STAT3 | |
Longo, Dan L.2  O'Shea, John J.3  Shimosaka, Akihiro1  Bacon, Chris M.3  Justin Tortolani, P.3  Rees, Robert C.4  | |
[1] Kirin Brewing Ltd., Tokyo, Japan;Laboratory of Leukocyte Biology, Biological Response Modifiers Program, NCI-FCRDC, Frederick, MD 21702, USA;Lymphocyte Cell Biology Section, Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Building 10/Room 9N262, National Institutes of Health, Bethesda, MD 20892, USA;Institute for Cancer Studies, University of Sheffield Medical School, Sheffield, South Yorkshire S10 2RX, UK | |
关键词: Thrombopoietin; Signal transduction; Signal transducers and activators of transcription (STAT); Tyrosine phosphorylation; | |
DOI : 10.1016/0014-5793(95)00796-C | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The growth and differentiation of megakaryocytes are regulated by thrombopoietin (TPO), a recently characterized cytokine which exerts its effects via a member of the hematopoietin receptor superfamily, c-Mpl. Since many cytokines which bind hematopoietin receptors activate the STAT family of transcription factors, we investigated whether STAT proteins were activated by TPO. TPO induced the formation of a DNA-binding complex recognizing a known STAT binding sequence. STAT5 was a major component of this DNA-binding complex, and STAT5 was tyrosine phosphorylated in response to TPO. Additionally, TPO-induced the tyrosine phosphorylation and DNA-binding activity of STAT3. Together with the recent demonstration of JAK2 activation in response to TPO, the data presented here define a rapid signaling pathway likely to be important in TPO-induced gene regulation.
【 授权许可】
Unknown
【 预 览 】
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