Effects of vasoactive substances and cAMP related compounds on adrenomedullin production in cultured vascular smooth muscle cells

FEBS Letters

Minamino, Naoto¹  Shoji, Hiroki¹  Kangawa, Kenji¹  Sugo, Shin¹  Matsuo, Hisayuki¹ 

[1] National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565, Japan

关键词: Adrenomedullin;    cAMP;    Vasoactive peptide;    Blood vessel;    Thrombin;    Rat vascular smooth muscle cell;   

DOI  :  10.1016/0014-5793(95)00769-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To elucidate the regulation mechanism of adrenomedullin (AM) production in blood vessels, we examined the effects of 30 substances on AM production in cultured rat vascular smooth muscle cells (VSMCs). Forskolin and 8-bromo-cAMP suppressed production and gene transcription of AM. Since VSMC expresses AM receptors coupled with adenylate cyclase, AM production may be regulated by intracellular cAMP concentration. Thrombin, vasoactive intestinal polypeptide and interferon-gg also inhibited AM production, while angiotensin II, endothelin-1, bradykinin, substance P, adrenaline, phorbol ester and fetal calf serum stimulated AM production in VSMC. These results suggest that AM production is regulated by a variety of substances, indicating complex systems regulating AM production.

【 授权许可】

Unknown