Differential expression of voltage‐activated Na+ currents in two prostatic tumour cell lines: contribution to invasiveness in vitro

FEBS Letters

Foster, C.S.¹  Fraser, S.P.²  Stephens, G.J.²  Downing, J.E.G.²  Laniado, M.E.²  Djamgoz, M.B.A.²  Abel, P.D.³  Grimes, J.A.² 

[1] University of Liverpool, Department of Pathology, Duncan Building, Liverpool L69 3BX, UK;Imperial College of Science, Technology and Medicine, Department of Biology, Neurobiology Group, London SW7 2BB, UK;Royal Postgraduate Medical School, Department of Surgery, London W12,ONN, UK

关键词: Ion channels;    Tetrodotoxin;    Prostate cancer;    Invasion;    Metastasis;    Electrophysiology;   

DOI  :  10.1016/0014-5793(95)00772-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The voltage-gated ionic currents of two rodent prostatic cancer cell lines were investigated using the whole-cell patch clamp technique. The highly metastatic Mat-Ly-Lu cells expressed a transient, inward Na⁺ current (blocked by 600 nM tetrodotoxin), which was not found in any of the weakly metastatic AT-2 cells. Although both cell lines expressed a sustained, outward K⁺ current, this occurred at a significantly higher density in the AT-2 than in the Mat-Ly-Lu cells. Incubation of the Mat-Ly-Lu cell line with 600 nM tetrodotoxin significantly reduced the invasive capacity of the cells in vitro. Under identical conditions, tetrodotoxin had no effect on the invasiveness of the AT-2 cells.

【 授权许可】

Unknown