Identification of κ‐ and δ‐opioid receptor transcripts in immune cells

FEBS Letters

Peluso, Jean²  Gavériaux, Claire²  Laforet, Jean¹  Kieffer, Brigitte²  Simonin, Fréderic² 

[1] Etablissement de Transfusion Sanguine, Laboratoire d'histocompatibilité, 67085 Strasbourg Cedex, France;Ecole Supérieure de Biotechnologie de Strasbourg, Bvd Sébastien Brandt, 67400 Illkirch, France

关键词: Opioid receptor transcript;    Opioid receptor (δ;    μ;    κ);    Reverse transcriptase-polymerase chain reaction;    Immune cell;    cDNA;    complementary DNA;    FCS;    fetal calf serum;    PBL;    peripheral blood lymphocytes;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    Tm;    putative transmembrane domain;   

DOI  :  10.1016/0014-5793(95)00766-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To investigate the role of opioids as direct modulators of the immune response, we have searched for expression of the recently cloned δ, μ and κ opioid receptors in immune cells. We have devised a reverse transcriptase-polymerase chain reaction strategy which specifically detects a region spanning putative transmembrane regions 2 to 7 for each transcript in both human and mouse immune cells. In human peripheral blood lymphocyte and monocyte preparations, δ was undetectable while the κ transcript was present. The analysis of human cell lines revealed low but significant levels of δ opioid receptor transcripts in T, B or monocyte cell lines while the κ transcript was found in B cell lines only. Investigation of murine cells showed the presence of transcript for the δ receptor in splenocytes and in some T and B cell lines. Unexpectedly, no expression of the μ receptor was detected. Sequence analysis of PCR products demonstrated nucleotide identity between immune and neuronal transcripts, indicating that they derive from the same genes. In conclusion, our results lead to the identification of κ and δ opioid receptor transcripts in immune cells.

【 授权许可】

Unknown