Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion

FEBS Letters

Tachikawa, Hiroyuki²  Miura, Tadashi¹  Takeuchi, Yutaka¹  Gao, Xiao-Dong¹  Fujimoto, Daisaburo²  Funahashi, Wataru¹  Onodera, Kazukiyo¹  Mizunaga, Takemitsu¹ 

[1] Division of Agriculture and Agricultural Life Science, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113, Japan;Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwaicho, Fuchushi, Tokyo 183, Japan

关键词: Protein disulfide isomerase;    Multicopy suppressor;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(95)00750-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MPD1, a yeast gene the overexpression of which suppresses the inviability caused by the loss of protein disulfide isomerase (PDI) was isolated and characterized. The MPD1 gene product retained a single disulfide isomerase active site sequence (APWCGHCK), an N-terminal putative signal sequence, and a C-terminal endoplasmic reticulum (ER) retention signal, and was a novel member of the PDI family. The gene product, identified in yeast extract, contained core size carbohydrates. MPD1 was not essential for growth, but overexpression of the gene suppressed the maturation defect of carboxypeptidase Y caused by PDI1 deletion, indicative of the related function to PDI in the yeast ER.

【 授权许可】

Unknown