Gamma‐glutamyltransferase expression during all‐trans retinoic acid‐induced differentiation of hematopoietic cell lines

FEBS Letters

Leh, Hervé¹  El yaagoubi, Mohamed¹  Hachad, Houda¹  Wellman, Maria¹  Siest, Gérard¹ 

[1] Centre du Médicament. URA CNRS 597, 30 rue Lionnois, 54000 Nancy, France

关键词: Differentiation;    Gamma-glutamyltransferase;    Hematopoietic cell line;    Retinoic acid;    Retinoid;    GGT;    gamma-glutamyltransferase;    PBS;    phosphate-buffered saline;    RA;    all-trans retinoic acid;    DMSO;    dimethylsulfoxide;    NBT;    nitro-blue-tetrazolium;    TPA;    12-0-tetradecanoylphorbol-13-acetate;    MTT;    (3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyl tetrazolium bromide);    APL;    acute promyelocytic leukemia;    ALL;    acute lymphoblastic leukemia;    AML;    acute myeloid leukemia;    MDS;    myelodysplasic syndrom;   

DOI  :  10.1016/0014-5793(95)00753-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gamma-glutamyltransferase activity, genes transcripts and differentiation by all-trans retinoic acid have been investigated in cultured HL-60, U937 and K562 cells. Acquisition of morphological and functional characteristics confirmed the terminal differentiation of HL-60 and U937 cells. All-trans r retinoic acid increased gamma-glutamyltransferase activity in a cell type- and time-dependent manner. Treatments with all-trans retinoic acid isomers and structurally analogs showed that only retinoids with carboxylic acid group were able to induce enzyme activity in terminal differentiated cells. Additionally, the analysis of gamma-glutamyltransferase genes transcription products demonstrated clearly that, both in untreated and in RA treated cells, only mRNA type I transcribed from the gene 6, was expressed.

【 授权许可】

Unknown