Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae

FEBS Letters

Attenborough, Sheila¹  Goderis, Inge²  De Samblanx, Genoveva W.²  Broekaert, Willem F.²  Osborn, Rupert W.¹  Torrekens, Sophie³  Thevissen, Karin²  Van Leuven, Fred³  Rees, Sarah B.¹ 

[1] Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berks, UK;F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, Belgium;Center of Human Genetics, Catholic University of Leuven, Willem De Croylaan 42, B-3001 Heverlee, Belgium

关键词: Peptide;    Amino acid sequence;    γ-Thionin;    Antifungal;    Antimicrobial;    AFP;    antifungal protein;    AMP;    antimicrobial protein;    Ah;    Aesculus hippocastanum;    Ct;    Clitoria ternatea;    Dm;    Dahlia merckii;    Hs;    Heuchera sanguinea;    Rs;    Raphanus sativus;    IC50;    protein concentration required for 50% growth inhibition;    RP-HPLC;    reversed-phase high-performance liquid chromatography;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(95)00666-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From seeds of Aesculus hippocastanum, Clitoria ternatea, Dahlia merckii and Heuchera sanguinea five antifungal proteins were isolated and shown to be homologous to plant defensins previously characterised from radish seeds and γ-thionins from Poaceae seeds. Based on the spectrum of their antimicriobial activity and the morphological distortions they induce on fungi the peptides can be divided into two classes. The peptides did not inhibit any of three different α-amylases.

【 授权许可】

Unknown