The γ subunit in the Escherichia coli ATP synthase complex (ECF1F0) extends through the stalk and contacts the c subunits of the F0 part

FEBS Letters

Watts, Spencer D.²  Capaldi, Roderick A.²  Zhang, Ying¹  Fillingame, Robert H.¹ 

[1] Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, WI 53706, USA;Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA

关键词: F1F0 ATPase;    c Subunit mutant;    Cross linking;    γ and ε Subunits;   

DOI  :  10.1016/0014-5793(95)00658-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A mutant, in which a cysteine has been site-directed into the polar loop region of the c subunit at residue 44, has been studied. Cross-linking of the c subunit to both the γ and ϵ subunits was observed with cupric 1,10-phenanthrolinate treatment. The linkage between the c and γ subunits was localized to that part of the γ subunit between residues 202–286, based on peptide analysis. Reference to the high resolution structure of F₁ [Abrahams et al. (1994) Nature 370, 621–628] appears to limit this contact site to the region including residues 202–230. This segment contains 4 tyrosines and 1 tryptophan as possible reactive residues for cross-linking with the c subunit cysteine.

【 授权许可】

Unknown