The structure of porcine protegrin genes

FEBS Letters

Ganz, Tomas¹  Lehrer, Robert I.¹  Zhao, Chengquan¹ 

[1] Department of Medicine, UCLA School of Medicine, UCLA Center for the Health Sciences, CHS 37-055, 10833 LeConte Avenue, Los Angeles, CA 90095-1678, USA

关键词: Protegrin;    Gene structure;    Antibiotic peptide;    PG-5;   

DOI  :  10.1016/0014-5793(95)00633-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned the genes of three protegrins, a family of cathelin-associated antimicrobial peptides originally isolated from porcine leukocytes. Each gene comprised 4 exons and 3 introns, wherein Exon I encoded the signal sequence and the first 37 amino acids of cathelin, Exons II and III contained 36 and 24 additional cathelin residues and Exon IV contained the final two cathelin residues followed by the protegrin sequence. This quadripartite gene structure helps explain how structurally diverse antimicrobial peptides can be expressed on common, cathelin-containing precursors. Southern blot probed with an oligonucleotide specific for protegrin genes suggested that several identical or nearly identical protegrin genes were densely clustered in the pig chromosome.

【 授权许可】

Unknown