Insulin and insulin‐like growth factor I stimulate expression of the primary response gene cMG1/TIS11 b by a wortmannin‐sensitive pathway in RIE‐1 cells

FEBS Letters

Brown, Kenneth D.¹  Corps, Anthony N.¹ 

[1] Department of Cellular Physiology, AFRC Babraham Institute, Babraham Hall, Babraham, Cambridge CB2 4AT, UK

关键词: Insulin;    Primary response gene;    Phosphatidylinositol 3-kinase;    Mitogen-activated protein kinase;    RIE-1 epithelial cell;    AII;    angiotensin II;    EGF;    epidermal growth factor;    GAPDH;    glyceraldehyde phosphate dehydrogenase;    IBMX;    3-isobutyl-1-methylxanthine;    IGF;    insulin-like growth factor;    MAP kinase;    mitogen-activated protein kinase;    PI3K;    phosphatidylinositol 3-kinase;    PI-3;    4;    5-P3;    phosphatidylinositol 3;    4;    5-trisphosphate;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoyl phorbol-13-acetate;    WM;    wortmannin;   

DOI  :  10.1016/0014-5793(95)00635-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of insulin or insulin-like growth factor I (IGF-I) to RIE-1 cells increased the expression of the primary response gene cMG1; dose-response analysis suggested that this effect was mediated largely through type 1 IGF receptors. Insulin/IGF-I did not affect the expression of the cMG1-related genes TIS11 and TIS11d, whereas epidermal growth factor, angiotensin II or 12-O-tetradecanoyl expression of all three genes. Incubation with wortmannin (WM) prevented the insulin/IGH-I-induced elevation of cMG1 mRNA, but not that induced by the other mitogens or the stimulation of mitogen-activated protein kinase by insulin. We conclude that WM-sensitive phosphatidylinositol 3-kinase may be involved in the specific stimulation of cMG1 expression by insulin/IGF-I.

【 授权许可】

Unknown