The mode of actions of glyceraldehyde‐3‐phosphate dehydrogenase identified as an immunoglobulin production stimulating factor

FEBS Letters

Sasaki, Takeshi¹  Sugahara, Takuya¹  Shirahata, Sanetaka²  Murakami, Hiroki² 

[1] Department of Biotechnology, College of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790, Japan;Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka 812, Japan

关键词: Glyceraldehyde-3-phosphate dehydrogenase;    Human Burkitt's lymphoma Namalwa cells;    Human-human hybridoma;    Immunoglobulin production stimulating factor;    Serum-free medium;   

DOI  :  10.1016/0014-5793(95)00607-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoglobulin production stimulating factor-IIα, which enhances immunoglobulin production of human and mouse hybridomas was purified from cell lysate of human Burkitt's lymphoma, Namalwa cells, and identified as glyceraldehyde-3-phosphate dehydrogenase. The enhancement of immunoglobulin production with this enzyme was not linked with its enzymatic activity. The enzyme enhanced immunoglobulin productivity of transcription-suppressed hybridomas, but did not enhance that of translation-suppressed hybridomas. From these results, it is suggested that this enzyme takes part in the post-translational control or the enhancement of translation activity to stimulate immunoglobulin production of hybridomas.

【 授权许可】

Unknown