AP‐1 DNA‐binding activity is inhibited by selenite and selenodiglutathione

FEBS Letters

Spyrou, Giannis¹  Kumar, Sushil¹  Holmgren, Arne¹  Björnstedt, Mikael¹ 

[1] The Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 77 Stockholm, Sweden

关键词: Selenium;    Selenodiglutathione;    Redox regulation;    AP-1;    AP-1;    activator protein-1;    GS-Se-SG;    selenodiglutathione;    Trx;    thioredoxin;    TR;    thioredoxin reductase;    SeO3 2−;    selenite;    SeO4 2−;    selenate;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(95)00599-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of the transcription factor AP-1 to DNA has been shown to be modulated by redox control mechanisms. Selenite and selenodiglutathione (GS-Se-SG), inhibit mammalian cell growth and are efficient oxidants of reduced thioredoxin and reduced thioredoxin reductase. Here, we report that selenite and GS-Se-SG efficiently inhibited AP-1 DNA-binding in nuclear extracts from 3B6 lymphocytes. A GS-Se-SG concentration of 0.75 μM resulted in 50% inhibition of AP-1 DNA-binding, whereas the same effect was achieved with 7.5 μM selenite. Nuclear extracts prepared from human 3B6 lymphocytes exposed for 4 h to 10 μM selenite showed a 50% reduction of AP-1 binding. These data suggest that selenite and selenodiglutathione inactivate the AP-1 factor and provide a mechanism by which selenium compounds inhibit cell growth.

【 授权许可】

Unknown