Intracellular transport of inositol‐containing sphingolipids in the yeast, Saccharomyces cerevisiae

FEBS Letters

Hechtberger, Petra¹  Daum, Günther¹ 

[1] Institut für Biochemie und Lebensmittelchemie, Technische Universität Graz Petersgasse 1212, A-8010 Graz, Austria

关键词: Sphingolipid;    Lipid transport;    Protein secretion;    Plasma membrane;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(95)00567-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Organelles of the early protein secretion pathway (ER, Golgi) are involved in biosynthesis and intracellular migration of the yeast sphingolipids, inositolphosphorylceramide (IPC), mannosylinositolphosphorylceramide (MIPC), and mannosyldiinositolphosphorylceramide (M(IP)₂C). Cycloheximide and nocodazole neither block biosynthesis of sphingolipids, nor ER to Golgi transport of IPC. In contrast, treatment of yeast cells with brefeldin A, which affects integrity of the Golgi, decreases formation of IPC and MIPC. Interruption of late steps of protein secretion (Golgi to plasma membrane transport) in temperature-sensitive secretory mutants prevents sphingolipids from being transported to the cell periphery.

【 授权许可】

Unknown