Lack of correlation between mRNA expression and enzymatic activity of the aspartate aminotransferase isoenzymes in various tissues of the rat

FEBS Letters

Doonan, S.¹  Perlino, E.¹  Marra, E.¹  Greco, M.¹  Abruzzese, F.¹ 

[1] Centro Studi Mitocondri e Metabolismo Energetico, C.N.R., Via Amendola 1651A, I-70126 Bari, Italy

关键词: Aspartate aminotransferase;    Isoenzyme;    Enzyme regulation;    mRNA regulation;    mRNA expression;   

DOI  :  10.1016/0014-5793(95)00518-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Little is known about control of expression of basal levels of the aspartate aminotransferases which are ubiquitous ‘house keeping’ enzymes in vertebrates. We have measured both mRNA and activity levels for both isoenzymes in various rat tissues as a function of age. Patterns of mRNA expression for the two isoenzymes were similar in a particular tissue about differed widely between tissues. Surprisingly, there was no simple correlation between mRNA levels and specific activities of the enzyme products. We conclude that translation for mRNA for these two isoenzymes is subject to tissue-specific, and in some cases age-related, regulation.

【 授权许可】

Unknown