Cloning and controlled overexpression of the gene encoding the 35 kDa soluble lytic transglycosylase from Escherichia coli

FEBS Letters

Hermann, Francis¹  Dijkstra, Arnoud J.¹  Keck, Wolfgang¹ 

[1] Pharma Research Department, Hoffmann-La Roche Ltd., CH-4002 Basel, Switzerland

关键词: Lytic transglycosylase;    Peptidoglycan metabolism;    Lysozyme;    Eschirichia coli;   

DOI  :  10.1016/0014-5793(95)00505-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lytic transglycosylases of Escherichia coli are involved in peptidoglycan metabolism and resemble the lysozymes not only in activity, but in the case of the 70 kDa soluble lytic transglycosylase (Slt70), also structurally. Here we report the cloning of the gene that encodes the 35 kDa soluble lytic transglycosylase (Slt35) of E. coli. Based on the sequence of the full-length gene, Slt35 is very likely to be a proteolytically truncated form of a slightly large protein. The homology between Slt35 and Slt70, albelt poor, indicates that the active site architecture of both proteins may be alike. Using the T-7 promoter system, Slt35 was overproduced in large quantities and purified to homogeneity for crystallographic purposes.

【 授权许可】

Unknown