Properties of ω conotoxin MVIIC receptors associated with α1A calcium channel subunits in rat brain

FEBS Letters

Leveque, Christian¹  Takahashi, Masami²  Seagar, Michael¹  Sato, Kazuki²  Martin-Moutot, Nicole¹  Kato, Rika² 

[1] INSERM U374, Institut Jean Roche, Faculté de Médecine Secteur Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France;Mitsubishi Kasei Institute of Life Science, Machida, Tokyo 194, Japan

关键词: Calcium channel;    ω conotoxin MVIIC receptor;    α1A subunit;    α2 subunit;    ωGVIA;    ω conotoxin GVIA;    ωMVIIC;    ω conotoxin MVIIC;    ωAgaIVA;    ω agatoxin IVA;    DSS;    disuccinimidyl suberate;    BSA;    bovine serum albumin;    TBS;    Tris-buffered saline;    CHAPS;    (3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane-sulfonate);    DHP;    1;    4-dihydropyridine;   

DOI  :  10.1016/0014-5793(95)00467-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Solubilized ¹²⁵I-ω conotoxin MVIIC receptors from rat cerebellum were immunoprecipitated by antibodies directed against the calcium channel α1A subunit. Anti-αIA antibodies recognized a 240-220, 180 and 160 kDa proteins in immunoblots of cerebellar membranes. Disuccinimidyl suberate cross-linked ¹²⁵I-ω conotoxin MVIIC to an α2δ-like 200-180 kDa subunit, which migrated at 150-140 kDa after disulfide reduction. These observations are consistent with a heteromeric structure in which high affinity ω conotoxin MVIIC binding sites formed by α1A subunits are located in close proximity to peripheral α2 subunits.

【 授权许可】

Unknown