Human thrombin variable region 1, including E39, is involved in interactions with α1‐antitrypsin M358R and protein C

FEBS Letters

Gaussem, Pascale¹  Arnaud, Emmanuel¹  Picard, Véronique¹  Aiach, Martine¹  Chadeuf, Gilliane¹ 

[1] INSERM U.428, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris V, 4 avenue de l'Observatoire, F-75270 Paris Cedex 06, France

关键词: Thrombin;    Variable region;    Autoantibody;    Protein C;    R358 α1-antitrypsin;   

DOI  :  10.1016/0014-5793(95)00457-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We used an antithrombin autoantibody (IgG D), the epitope of which encompasses ABE1 and amino acids located within variable region 1, to study thrombin interactions with R358 α1-AT and protein C. IgG D inhibited the thrombin interaction with R358 α1-AT, while hirugen had no effect, indicating that the interaction of R358 α1-AT with thrombin may involve the VR1 subsite. We also obtained evidence that VR1 may be involved in the activation of protein C by thrombin in the absence of thrombomodulin. Moreover, IgG D attenuated the inhibitory effect of calcium ions during protein C activation by thrombin, probably by masking E 39 within the VR1 site.

【 授权许可】

Unknown