FEBS Letters | |
Human thrombin variable region 1, including E39, is involved in interactions with α1‐antitrypsin M358R and protein C | |
Gaussem, Pascale1  Arnaud, Emmanuel1  Picard, Véronique1  Aiach, Martine1  Chadeuf, Gilliane1  | |
[1] INSERM U.428, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris V, 4 avenue de l'Observatoire, F-75270 Paris Cedex 06, France | |
关键词: Thrombin; Variable region; Autoantibody; Protein C; R358 α1-antitrypsin; | |
DOI : 10.1016/0014-5793(95)00457-K | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We used an antithrombin autoantibody (IgG D), the epitope of which encompasses ABE1 and amino acids located within variable region 1, to study thrombin interactions with R358 α1-AT and protein C. IgG D inhibited the thrombin interaction with R358 α1-AT, while hirugen had no effect, indicating that the interaction of R358 α1-AT with thrombin may involve the VR1 subsite. We also obtained evidence that VR1 may be involved in the activation of protein C by thrombin in the absence of thrombomodulin. Moreover, IgG D attenuated the inhibitory effect of calcium ions during protein C activation by thrombin, probably by masking E 39 within the VR1 site.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020301145ZK.pdf | 345KB | download |