Identification of Rab3‐, Rab5a‐ and synaptobrevin II‐like proteins in a preparation of rat kidney vesicles containing the vasopressin‐regulated water channel

FEBS Letters

Liebenhoff, Ursula¹  Rosenthal, Walter¹ 

[1] Rudolf-Buchheim-Institut für Pharmakologie, Justus-Liebig-Universität Gießen, Frankfurter Str. 107, D-35392 Gießen, Germany

关键词: Antidiuresis;    Water channel;    AQP-CD;    Exocytosis;    Small GTP-binding protein;    Synaptobrevin;    AQP-CD;    aquaporin-collecting duct;    GTPS;    guanosine 5′-[γ-thio]triphosphate;   

DOI  :  10.1016/0014-5793(95)00476-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

According to the ‘shuttle hypothesis’, vasopressin increases the water permeability of renal epithelial cells by exocytotic fusion of vesicles containing the water channel AQP-CD with the apical plasma membrane, whereas withdrawal of vasopressin results in endocytotic uptake of AQP-CD. The proteins involved in the redistribution of AQP-CD have not been identified. With a panel of monoclonal antibodies, we detected Rab3-, Rab5a- and synaptobrevin II-like proteins in a kidney preparation enriched in AQP-CD-containing vesicles. The synaptobrevin II-like protein is not identical with the ubiquitous cellubrevin. Rab3- and synaptobrevin II- but not Rab5a-like proteins were co-enriched with AQP-CD. The data suggest that the proteins involved in hormonal regulation of water permeability in kidney epithelial cells are identical or similar to those involved in regulated exocytosis in secretory cells.

【 授权许可】

Unknown