Rev‐dependency of expression of human immunodeficiency virus type 1 gag and env genes

FEBS Letters

Adachi, Akio¹  Furuta, Rika A.¹  Hatanaka, Masakazu¹  Kawamura, Meiko¹  Sakai, Hiroyuki¹  Tokunaga, Kenzo¹ 

[1] Institute for Virus Research, Kyoto University, Kyoto 606, Japan

关键词: HIV-1;    Rev-dependency;    gag;    env;    nef;   

DOI  :  10.1016/0014-5793(95)00444-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and singly-spliced viral mRNAs was demonstrated to be differentially dependent on the Rev function. This difference of Rev-dependency was found not to be determined by cis-elements in gag, pol, and env coding sequences reported so far, and was lost when the reporter constructs containing minimum elements for Rev-responsiveness such as splice signals and rev responsive element were used for experiments. These findings indicated that fundamental structure of HIV-1 mRNA was critical for the differential regulation of gene expression by Rev transactivator.

【 授权许可】

Unknown