Synaptotagmin I‐ and II‐deficient PC12 cells exhibit calcium‐independent, depolarization‐induced neurotransmitter release from synaptic‐like microvesicles

FEBS Letters

Huttner, Wieland B.¹  Bauerfeind, Rudolf¹  Jelinek, Ruth¹ 

[1] Institute for Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany

关键词: Calcium;    Exocytosis;    Regulated secretion;    Secretory granule;    Synaptic vesicle;    Synaptotagmin;    SLMV;    synaptic-like microvesicle;    SgII;    secretogranin II;   

DOI  :  10.1016/0014-5793(95)00419-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synaptotagmin I- and II-deficient PC12 cells (ShojiKasai et al. [1]) were used to compare the role of this protein in the calcium-dependent exocytosis of secretory granules and synaptic-like microvesicles (SLMVs). While neither catecholamine nor protein secretion from secretory granules were altered, the depolarization-induced acetylcholine release from SLMVs was no longer calcium-dependent. We propose that within the exocytotic process of SLMVs, there exist two depolarization-induced steps. One is calcium-dependent and no longer present in synaptotagmin I- and II-deficient cells. The other is induced by depolarization, does not require calcium, and suffices to trigger neurotransmitter release from SLMVs in synaptotagmin I- and II-deficient PC12 cells.

【 授权许可】

Unknown