Peroxynitrite‐mediated oxidative protein modifications

FEBS Letters

Ischiropoulos, Harry¹  Al-Mehdi, Abu B.¹ 

[1] Institute for Environmental Medicine, University of Pennsylvania, School of Medicine Philadelphia, Pennsylvania 19104, USA

关键词: Nitrotyrosine;    Dityrosine;    Protein carbonyl;    Tryptophan oxidation;    Protein fragmentation;   

DOI  :  10.1016/0014-5793(95)00307-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins are targets of reactive species and detection of oxidatively modified proteins is often used as an index of oxidative stress. Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide. Using fatty acid-free bovine serum albumin as a model we examined peroxynitrite-mediated protein modifications. The reaction of protein with peroxynitrite resulted in the oxidation of tryptophan and cysteine, in the nitration of tyrosine, in the formation of dityrosine, in the production of 2,4 dinitrophenylhydrazine-reactive carbonyls and in protein fragmentation. The formation of 3-nitrotyrosine represents a specific peroxynitrite-mediated protein modification that is different from modifications mediated by reactive oxygen species.

【 授权许可】

Unknown