Protein histidine phosphatase activity in rat liver and spinach leaves

FEBS Letters

MacKintosh, Carol¹  Matthews, Harry R.¹ 

[1] MRC Protein Phosphorylation Unit, Biochemistry Department, The University, Dundee DD1 4HN, UK

关键词: Phosphoprotein phosphatase;    Protein histidine phosphatase;    Phosphohistidine;    Rat;    Spinach;   

DOI  :  10.1016/0014-5793(95)00353-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Whole cell extracts from rat liver or spinach leaves contain divalent ion-independent protein histidine phosphatase activity due to phosphatases of the PP1/PP2A family. In the rat liver extract, almost all the activity was found in the PP1, PP2A₁ and PP2₂ peaks. In the spinach leaf extract, four phosphorylase phosphatase activity peaks were resolved — three containing PP1 and one containing PP2A — and all showed histidine phosphatase activity. Thus, protein histidine phosphatase activity is expressed in the cytosolic forms of protein phosphatases of the PP1/PP2A family in mammalian and plant cells.

【 授权许可】

Unknown