Expression of protein kinase C isozymes in hippocampal neurones in culture

FEBS Letters

Rodríguez-Sánchez, Paloma¹  Tejero-Díez, Pedro¹  Javier Díez-Guerra, F.¹ 

[1] Centro de Biología Molecular, Facultad de Ciencias Universidad Autónoma de Madrid, 28049 Madrid, Spain

关键词: PKC;    Isozyme;    Neuron;    Differentiation;    Proteolysis;    Hippocampus;   

DOI  :  10.1016/0014-5793(95)00303-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes α, β and γ, that are expressed at very low levels during the initial stages and then increase continuously with time of culture. Secondly, novel PKC isozymes δ, ϵ and ζ, whose contents increase very early to reach a maximum after three days of culture and then progressively decline. Specific proteolysis for PKC isozymes β and γ was observed throughout the period studied. The developmental profile obtained for the different PKC isozymes is discussed in relation to the differentiation of hippocampal neurones in culture.

【 授权许可】

Unknown