The transcriptional regulation of human arachidonate 12‐lipoxygenase gene by NFκB/Rel

FEBS Letters

Yamamoto, Shozo¹  Yoshimoto, Tanihiro¹  Arakawa, Toshiya¹  Nakamura, Michihiro¹ 

[1] Department of Biochemistry, Tokushima University, School of Medicine, Kuramoto-cho, Tokushima 770, Japan

关键词: 12-Lipoxygenase;    Transcription factor;    NFκB;    Rel;    HEL;    human erythroleukemia;    EMSA;    electrophoretic mobility-shift assay;    PMSF;    phenylmethylsulfonyl fluoride;    bp;    base pair(s);    FCS;    fetal calf serum;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/0014-5793(95)00293-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As examined by the luciferase assay, a negative regulatory region including the NFκB motif was found in the 5′-flanking region of the 12-lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site-specific mutation of the NFκB motif. Probes including the NFκB region gave positive bands upon a gel-shift assay. The bands were supershifted by antibodies for NFκB p50, NFκB p65 and c-Rel, and were lost by a NFκB competitor DNA. Furthermore, the NFκB sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c-Rel) seemed to control the over-expression of the human 12-lipoxygenase gene.

【 授权许可】

Unknown