Molecular cloning and expression of subunit 12: a non‐MCP and non‐ATPase subunit of the 26 S protease

FEBS Letters

Dubiel, Wolfgang¹  Dumdey, Renate¹  Ferrell, Katherine²  Prehn, Siegfried¹  Rechsteiner, Martin²  Standera, Sybille¹ 

[1] Institut für Biochemie, Universitätsklinikum Charité, Humboldt-Universität, Hessische Str. 3–4, 10115 Berlin, Germany;Department of Biochemistry, University of Utah, School of Medicine, Salt Lake City, UT 84132, USA

关键词: Human 26 S protease;    Regulatory complex;    Subunit 12;    Mov-34;   

DOI  :  10.1016/0014-5793(95)00288-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding subunit 12 (S12) of human erythrocyte 26 S protease has been isolated, sequenced and expressed. The cDNA contains an open reading frame that encodes a 36.6 kDA protein 96% identical to mouse Mov-34 and 67% identical to its Drosophila melanogaster homolog. Based on the high degree of sequence identity between human S12, mouse and Drosophila Mov-34 proteins, we conclude that the Mov-34 gene product is a component of the 26 S protease. Antibodies produced against two S12 fragments, Met¹-Tyr⁹⁵ (S12_f95) and Met¹-Leu²⁰⁵ (S12_f205), react with S12 transferred to nitrocellulose from SDS-PAGE. In contrast, after transfer from native gels, the epitope(s) recognized by anti-S12_f205 is exposed in the regulatory complex but appears to be masked when the regulatory complex associates with the multicatalytic protease.

【 授权许可】

Unknown