Site‐specific 15N‐labelling of oligonucleotides for NMR: the trp operator and its interaction with the trp repressor

FEBS Letters

Xu, Yao-Zhong¹  Ramesh, Vasudevan²  Roberts, Gordon C.K.² 

[1] Cancer Research Campaign Nitrosamine-induced Cancer Research Group, Department of Biochemistry and Molecular Biology, University College London, London, UK;Department of Biochemistry and Biological NMR Centre, Medical Science Building, University of Leicester, University Road, Leicester, UK

关键词: NMR;    Stable isotope;    Protein-DNA interaction;   

DOI  :  10.1016/0014-5793(95)00226-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A convenient and economical method is described for the site-specific ¹⁵N-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using ¹H-¹⁵N HMQC and ¹⁵N-edited ¹H-¹H NOESY experiments.

【 授权许可】

Unknown