Complete sequence and characterization of the major sperm nuclear basic protein from Mytilus trossulus

FEBS Letters

Rocchini, Corinne¹  Ausio, Juan¹  Rice, Philip¹ 

[1] Department of Biochemistry and Microbiology, University of Victoria, P.O. Box 3055, Victoria, BC, V8 W 3P6, Canada

关键词: Sperm nuclear basic protein;    Protein characterization;    Mytilus;    SNBP;    sperm nuclear basic protein;   

DOI  :  10.1016/0014-5793(95)00275-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have characterized the major protamine-like protein (PL-III) from the sperm of the mussel Mytilus trossulus. The molecular mass of the main protein component of PL-III, determined by mass spectrometry using fast atom bombardment and matrix-assisted laser desorption ionization was 11304 ± 6 Da. The complete protein sequence was established by Edman degradation and the molecular mass derived from this sequence coincides with that experimentally determined. The protein has a sedimentation coefficient s _20,w = 1.15 ± 0.5 S which is consistent with a random coil of radius of gyration 34.3 ± 1.4 A.

【 授权许可】

Unknown