Major allergen Phl p Vb in timothy grass is a novel pollen RNase

FEBS Letters

Schramm, G.¹  Keown, M.B.²  Becker, W.-M.¹  Bufe, A.¹  Schlaak, M.¹ 

[1] Research Institute Borstel, Allergology, Parkallee 22, D-23845 Borstel, Germany;The Randall Institute, King's College London, 26–29 Drury Lane, London, WC2B SRL, UK

关键词: Major allergen;    Timothy grass pollen;    cDNA;    RNase activity;    Salicylic acid;   

DOI  :  10.1016/0014-5793(95)00259-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA coding for the major group V allergen Phl p Vb was isolated from a timothy grass pollen cDNA library by immunoscreening with a specific monoclonal antibody. It was discovered for the first time that the recombinant Phl p Vb pollen allergen after expression and purification has ribonuclease activity. High homology of Phl p Vb to other group V allergens in grass pollen indicates similar function. By RNase activity gel of natural pollen extract of timothy grass and consecutive Western blot analysis of the excised proteins, the RNase active bands were shown to be group V allergens. Additionally it was demonstrated that an homologous protein to Phl p Vb in the mother plant could be induced by salicylic acid. This indicates that group Vb allergens may be involved in host-pathogen interactions because in pollen they are quickly exported RNases and in the mother plant they depend on a hormone which is related to expression of plant resistance genes.

【 授权许可】

Unknown