Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica

FEBS Letters

Raha, Sanghamitra¹  Giri, Banabihari¹  Bhattacharyya, Bhudeb²  Biswas, Birendra B¹ 

[1] Department of Biophysics, Molecular Biology & Genetics, Calcutta university, 92 APC Road, Calcutta-700009, India;Department of Biochemistry, Bose Institute, Calcutta-700054, India

关键词: Inositol(1;    4;    5)trisphosphate;    Inositol(1;    3;    4;    5)tetrakis-phosphate;    Calcium;    Entamoeba histolytica;    Fura-2;    EC50;    concentration causing half-maximal effect;    [Ca2+];    ambient free calcium concentration;   

DOI  :  10.1016/0014-5793(95)00265-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P₃)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P₄). Similarly, prior depletion of Ins(1,3,4,5)P₄-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P₃. The EC₅₀ for calcium release was 0.15 μM with Ins(1,4,5)P₃ and 0.68 μM with Ins(1,3,4,5)P₄. In conclusion, the Ins(1,3,4,5)P₄-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P₃-sensitive pool.

【 授权许可】

Unknown