Novel avidin and streptavidin binding sequences found in synthetic peptide libraries

FEBS Letters

Hansen, Per Hertz²  Holm, Arne³  Østergaard, Soren³  Olsen, Marianne¹ 

[1] Research Center for Medical Biotechnology, Protein Laboratory, University of Copenhagen, Panum Institute, 3C Blegdamsvej, 2200 Copenhagen N, Denmark;Novo Research Institute, Novo Allé, 2880 Bagsværd, Denmark;Research Center for Medical Biotechnology, Chemistry Department Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Copenhagen, Denmark

关键词: Synthetic peptide library;    Sub-library;    Avidin;    Streptavidin;    Molecular recognition;    BCIP;    5-bromo-4-chloro-3-indolyl phosphate;    BSA;    bovine serum albumin;    DAB;    diaminobenzidine;    DIEA;    diisopropylethylamine;    DMF;    N;    N-dimethylformamide;    EDT;    ethandithiol;    ELISA;    enzyme-linked immunosorbent assay;    Fmoc;    9-fluorenylmethoxycarbonyl;    HPLC;    high performance liquid chromatography;    HOBt;    1-hydroxybenzotriazole;    NBT;    nitro blue tetrazolium;    OPD;    o-phenylenediamine;    PBS;    phosphate-buffered saline;    Pfp;    pentafluorophenyl;    SPDP;    3-(2-Pyridyldithio)propionic acid N-hydroxy-succinimide ester;    TBTU;    2-(1H-benzotriazole-1-yl)-1;    1;    3;    3-tetramethyluronium tetrafluoroborate);    TFA;    trifluoracetic acid;   

DOI  :  10.1016/0014-5793(95)00262-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic resin-bound peptide libraries made of protein l-amino acids have been synthesized. During screening of the libraries, peptides that bind to avidin have been identified containing a novel motif with two histidines separated by one residue. A sub-library was synthesized and screened, and new critical residues appeared surrounding the two histidines. Additionally peptide libraries made of d-amino acids have been screened with avidin and streptavidin and novel motifs have been found.

【 授权许可】

Unknown