Iron release, membrane protein oxidation and erythrocyte ageing

FEBS Letters

Sugherini, L.²  Ferrali, M.²  Magnani, A.¹  Comporti, M.²  Ciccoli, L.²  Signorini, C.² 

[1] Department of Chemistry, University of Siena, Siena, Italy;Institute of General Pathology, University of Siena, Via del Laterino 8, I-53100 Siena, Italy

关键词: Erythrocyte;    Iron release;    Senescent cell antigen;    Protein oxidation;    Iron chelation;   

DOI  :  10.1016/0014-5793(95)00235-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aerobic incubation of erythrocytes in phosphate buffer for 24–60 h (a model of rapid in vitro ageing) induced progressive iron release and methemoglobin formation. Membrane proteins showed electrophoretic alterations and increase in carbonyl groups (as documented by IR spectroscopy). None of these phenomena were seen when the erythrocytes were incubated under anaerobic conditions. The membranes from aerobically incubated cells bound a much higher amount of autologous IgG than those from anaerobically incubated ones, suggesting that the aerobic incubation gives rise to the senescent antigen. The addition of ferrozine during the aerobic incubation prevented both the IgG binding and the protein alterations seen in the IR spectra, suggesting an intracellular chelation of the released iron by ferrozine.

【 授权许可】

Unknown