期刊论文详细信息
FEBS Letters | |
Improved measurement of 13C, 31P J coupling constants in isotopically labeled RNA | |
Pardi, Arthur1  Jucker, Fiona M.1  Legault, Pascale1  | |
[1] Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309-0215, USA | |
关键词: Heteronuclear NMR; RNA structure; J coupling constant; Backbone torsion angle; Ribozyme; | |
DOI : 10.1016/0014-5793(95)00232-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
3JCP coupling constants have been measured in 1 99% 13C, 15N labeled lead-dependent ribozyme, known as the leadzyme. These coupling constants were determined by analysis of the intensity of individual crosspeaks in a spin-echo difference constant time HSQC experiment. This procedure permits improved measurement of the 3JC2′P and 3JC4′P coupling constants in isotopically labeled RNA and yielded valuable information on the β and ϵ backbone torsion angles in the leadzyme.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020300890ZK.pdf | 492KB | download |