Improved measurement of 13C, 31P J coupling constants in isotopically labeled RNA

FEBS Letters

Pardi, Arthur¹  Jucker, Fiona M.¹  Legault, Pascale¹ 

[1] Department of Chemistry and Biochemistry, University of Colorado at Boulder, Boulder, CO 80309-0215, USA

关键词: Heteronuclear NMR;    RNA structure;    J coupling constant;    Backbone torsion angle;    Ribozyme;   

DOI  :  10.1016/0014-5793(95)00232-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

³J_CP coupling constants have been measured in 1 99% ¹³C, ¹⁵N labeled lead-dependent ribozyme, known as the leadzyme. These coupling constants were determined by analysis of the intensity of individual crosspeaks in a spin-echo difference constant time HSQC experiment. This procedure permits improved measurement of the ³J_C2′P and ³J_C4′P coupling constants in isotopically labeled RNA and yielded valuable information on the β and ϵ backbone torsion angles in the leadzyme.

【 授权许可】

Unknown