Equatorial split of holo‐chaperonin from Thermus thermophilus by ATP and K+

FEBS Letters

Sasabe, Hiroyuki²  Ishii, Noriyuki²  Taguchi, Hideki¹  Yoshida, Masasuke¹ 

[1] Laboratory of Resources Utilization R-1, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama 226, Japan;Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, Saitama 351-01, Japan

关键词: Chaperonin;    Chaperonin;    split of;    GroEL;    Thermus thermophilus;    cpn60;    chaperonin-60;    cpn10;    chaperonin-10;    holo-cpn;    holo-chaperonin;    HPLC;    high pressure liquid chromatography;    AMPPNP;    adenylylimidodiphosphate;   

DOI  :  10.1016/0014-5793(95)00222-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Holo-chaperonin molecule from Thermus thermophilus is a bullet-shaped particle whose cylinder part and round top are composed of two stacked rings of the cpn60 heptamer and a single ring of the cpn10 heptamer, respectively. We found that it splits at the plane between two cpn60 rings into two halves under physiological conditions, that is, in the presence of ATP (but not AMP-PNP, ADP) + K⁺ (but not Na⁺) at 60°C. This equatorial split could be functionally important although it has not been considered in any current mechanistic model of chaperonin functioning.

【 授权许可】

Unknown