The endozepine ODN stimulates polyphosphoinositide metabolism in rat astrocytes

FEBS Letters

Desrues, L.¹  Gandolfo, P.¹  Strijdveen, I.¹  Vaudry, H.¹  Tonon, M.C.¹  Lamacz, M.¹  Patte, C.¹ 

[1] European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France

关键词: Endozepine;    Octadecaneuropeptide;    Polyphosphoinositide metabolism;    Glial cell;    DBI;    diazepam-binding inhibitor;    ODN;    octadecaneuropeptide;    PTX;    pertussis toxin;    TTN;    triakontatetraneuropeptide;   

DOI  :  10.1016/0014-5793(95)00209-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Astrocytes synthesize a series of peptides called endozepines which act as endogenous ligands of benzodiazepine receptors. The present study demonstrates that the endozepine ODN causes a dose-dependent increase in inositol trisphosphate and a parallel decrease in phosphatidylinositol bisphosphate in cultured rat astrocytes. Pre-incubation of astrocytes with the phospholipase C inhibitor U 73122 or with pertussis toxin totally blocked polyphosphoinositide metabolism. These data show that, in rat astrocytes, ODN stimulates a phospholipase C coupled to a pertussis toxin-sensitive G protein.

【 授权许可】

Unknown