Diaminoalkanes with an odd number of carbon atoms induce compaction of a single double‐stranded DNA chain

FEBS Letters

Yoshikawa, Yuko¹  Yoshikawa, Kenichi¹ 

[1] Division of Informatics for Natural Sciences, Graduate School of Human Informatics, Nagoya University, Nagoya 464-01, Japan

关键词: Diaminoalkane;    Higher order structure of DNA;    Globular DNA;    Single molecular observation;   

DOI  :  10.1016/0014-5793(95)00190-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have performed direct observations on the conformational change of a single double-stranded T4 DNA molecule induced by diaminoalkanes, NH₃ ⁺(CH₂)_nNH₃ ⁺ (n = 1 ∼ 6), by use of fluorescence microscopy. It was found that diamines with three and five methylene groups show a significant effect on the compaction of individual single DNA molecules. On the other hand, diamines with two, four and six methylene groups are not effective in the compaction of DNA. Such a drastic difference in the action of diamines suggests that not only the number of charge but also the length between the amino groups in the diamines plays an essential role in their interactions with DNA.

【 授权许可】

Unknown