Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain

FEBS Letters

Letesson, Jean-Jacques¹  Zygmunt, Michel S.²  Tibor, Anne¹  Denoel, Philippe A.¹  Lichtfouse, Bernadette¹  Briffeuil, Pascal³  Weynants, Vincent¹  Limet, Joseph N.¹ 

[1]Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix, 61 rue de Bruxelles, B-5000 Namur, Belgium

[2]Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France

[3]Laboratoire de Biologie Quantitative, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium

关键词: Bacterioferritin;    Antigen A2;    Brucellosis;    Brucella melitensis;   

DOI  :  10.1016/0014-5793(95)00189-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 40 N-terminal amino acids of the 20 kDa antigen A2 from Brucella melitensis were sequenced and showed important similarities with 4 bacterioferritins. A monoclonal antibody raised against this antigen cross-reacted with Escherichia coli bacterioferritin. Hybridization of two sets of degenerate primers with B. melitensis HindIII-digested genomic DNA identified a 3.8 kb fragment. This fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. The sequence of the Brucella bacterioferritin is 69% similar to that of E. coli, and many of the ferroxidase centre and haem-ligation residues are conserved.

【 授权许可】

Unknown