FEBS Letters | |
Alternative polyadenylation generates three low‐pI α‐amylase mRNAs with differential expression in barley | |
Knauer, Nancy S.2  Skadsen, Ronald W.1  | |
[1] United States Department of Agriculture, Agriculture Research Service, Cereal Crops Research Unit, 501 N. Walnut St., Madison, WI 53705, USA;Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA | |
关键词: α-Amylase; Membrane-bound polysome; Alternative polyadenylation; Barley; | |
DOI : 10.1016/0014-5793(95)00112-M | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Specific low-pI α-amylase genes from barley (Hordeum vulgare L.) produced alternative mRNAs with a 17-base 3′ extension (extension 1) or a 17-base extension beyond this (extension 2). The extended mRNAs do not arise from splicing of downstream sequences, and not all low-pI genes contain the extended sequences. All three mRNAs occur in aleurones and shoots, while extension 2 is missing from scutella. Also, the unextended mRNAs predominate in total mRNA, but the extended mRNAs predominate in membrane-bound polysomes. The extended sequences do not occur in previously characterized α-amylases, but 16 of 18 bases, mainly in extension 1, are identical with a sequence in the 3′-UTR of PAPI, a putative inhibitor of α-amylase. These observations suggest that the extended sequences could play a functional role in α-amylase expression.
【 授权许可】
Unknown
【 预 览 】
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