Alternative polyadenylation generates three low‐pI α‐amylase mRNAs with differential expression in barley

FEBS Letters

Knauer, Nancy S.²  Skadsen, Ronald W.¹ 

[1] United States Department of Agriculture, Agriculture Research Service, Cereal Crops Research Unit, 501 N. Walnut St., Madison, WI 53705, USA;Department of Agronomy, University of Wisconsin, Madison, WI 53706, USA

关键词: α-Amylase;    Membrane-bound polysome;    Alternative polyadenylation;    Barley;   

DOI  :  10.1016/0014-5793(95)00112-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Specific low-pI α-amylase genes from barley (Hordeum vulgare L.) produced alternative mRNAs with a 17-base 3′ extension (extension 1) or a 17-base extension beyond this (extension 2). The extended mRNAs do not arise from splicing of downstream sequences, and not all low-pI genes contain the extended sequences. All three mRNAs occur in aleurones and shoots, while extension 2 is missing from scutella. Also, the unextended mRNAs predominate in total mRNA, but the extended mRNAs predominate in membrane-bound polysomes. The extended sequences do not occur in previously characterized α-amylases, but 16 of 18 bases, mainly in extension 1, are identical with a sequence in the 3′-UTR of PAPI, a putative inhibitor of α-amylase. These observations suggest that the extended sequences could play a functional role in α-amylase expression.

【 授权许可】

Unknown