Interaction of a synaptobrevin (VAMP)‐syntaxin complex with presynaptic calcium channels

FEBS Letters

Seagar, Michael J.¹  Leveque, Christian¹  Takahashi, Masami²  Charvin, Nathalie¹  El Far, Oussama¹  Martin-Moutot, Nicole¹ 

[1] INSERM U374, Institut Jean Roche, Faculté de Medécine Secteur Nord, Boulevard Pierre Dramard, 13916 Marseille Cedex 20, France;Mitsubishi Kasei Institute of Life Science, Machida, Tokyo 194, Japan

关键词: Neurotransmitter release;    Calcium channel;    Synaptobrevin;    Synaptophysin;    Syntaxin;    Synaptotagmin;   

DOI  :  10.1016/0014-5793(95)00156-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nerve terminal protein complexes implicated in exocytosis were examined by immuno-isolation from rat brain synaptosomes. Immunoprecipitation with anti-syntaxin or anti-VAMP antibodies revealed a syntaxin-SNAP25-VAMP-synaptotagmin complex. Anti-VAMP antibodies also trapped a distinct VAMP-synaptophysin complex. A similar fraction (about 70%) of N-type calcium channels ([¹²⁵I]ω conotoxin GVIA receptors), was immunoprecipitated by either anti-syntaxin or anti-VAMP antibodies, but not by anti-synaptophysin antibodies (< 4%). The majority of N- but not L-type calcium channels ([³H]PN200-110 receptors), appear to be associated with a synaptic vesicle prefusion complex.

【 授权许可】

Unknown