Wortmannin inhibits insulin‐stimulated but not contraction‐stimulated glucose transport activity in skeletal muscle

FEBS Letters

Holloszy, John O.¹  Lee, Abraham D.¹  Hansen, Polly A.¹ 

[1] Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA

关键词: Muscle;    Glucose transport;    Insulin;    Contraction;    Phosphatidylinositol 3-kinase;    PI 3-kinase;    phosphatidylinositol 3-kinase;    IRS-1;    insulin receptor substrate-1;    IGF-1;    insulin-like growth factor-1;    KHB;    Krebs-Henseleit buffer;    BSA;    bovine serum albumin;    DMSO;    dimethyl sulfoxide;    2-DG;    2-deoxy-d-glucose;   

DOI  :  10.1016/0014-5793(95)00147-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In skeletal muscle, glucose transport is stimulated by insulin, contractions and hypoxia. In this study, we used the phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor wortmannin to examine whether (i) PI 3-kinase activity is necessary for stimulation of glucose transport by insulin in muscle, and (ii) PI 3-kinase mediates a step in the pathway by which contractions/hypoxia stimulate glucose transport. Wortmannin completely blocked insulin- and insulin-like growth factor-1-stimulated glucose transport in muscle. In contrast, wortmannin had no effect on the stimulation of glucose transport by contractions or hypoxia, providing evidence that PI 3-kinase activity is not involved in the activation of glucose transport by these stimuli.

【 授权许可】

Unknown