The expression of esterase S gene of Drosophila virilis in Drosophila melanogaster

FEBS Letters

Pavlova, Galina V.²  Georgiev, Georgii P.³  Shostak, Natalia G.³  Sergeev, Pavel V.³  Kopantseva, Marina R.¹  Korochkin, Leonid I.²  Bashkirov, Victor N.²  Panin, Vladislav M.² 

[1] Institute of Developmental Biology, Russian Acad. Sci., 26 Vavilov str, 117984 Moscow, Russian Federation;Institute of Gene Biology, Russian Acad. Sci., 34/5 Vavilov str, 117334 Moscow, Russian Federation;Engelhardt Institute of Molecular Biology, Russian Acad Sci., 32 Vavilov str., 117984 Moscow, Russian Federation

关键词: Esterase;    Regulation of expression;    Drosophila virilis;    SDS;    sodium dodecyl sulfate;    BSA;    bovine serum albumin;    estS;    esterase S;    est6;    esterase 6;   

DOI  :  10.1016/0014-5793(95)00102-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Drosophila melanogaster was transformed with the esterase S gene from Drosophila virilis. This gene is strongly activated in ejaculatory bulbs of mature males of Drosophila virilis. The closely related gene from Drosophila melanogaster is activated in ejaculatory ducts. The tissue- and stage-specific expression of incomplete genomic copy of the esterase S gene integrated into the Drosophila melanogaster genome is the same as in Drosophila virilis. These data show that tissue and stage specificity is determined by relatively small 5′ regulatory region of the esterase S gene. The comparison between deduced amino-acid sequences of the esterase S of Drosophila virilis and esterase 6 of Drosophila melanogaster was performed. These sequences revealed 50% homology.

【 授权许可】

Unknown