mRNA encoding the translation initiation factor eIF‐4E is expressed early in Xenopus embryogenesis

FEBS Letters

Saigoh, Motoki¹  Shiokawa, Koichiro²  Miura, Kin-ichiro¹  Wakiyama, Motoaki¹ 

[1] Institute for Biomolecular Science, Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo, Japan;Zoological Institute, Faculty of Science, The University of Tokyo, Tokyo, Japan

关键词: Translation initiation factor;    eIF-4E;    mRNA;    5′-RACE;    RT-PCR;    Xenopus laevis;   

DOI  :  10.1016/0014-5793(95)00081-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The translation initiation factor eIF-4E plays an important role in regulating the overall rate of translation in eukaryotic cells. To investigate the expression of eIF-4E itseIF, we characterized the eIF-4E mRNA expressed in Xenopus embryos. 5′-RACE was performed to determine the 5′-end of the mRNA and the result predicts isoforms differing at the amino-terminal end. Expression of the eIF-4E mRNA in Xenopus oocytes and embryos was examined by RT-PCR. Xenopus eIF-4E mRNA is produced during oogenesis and persists during the early stages of embryogenesis as a maternal mRNA.

【 授权许可】

Unknown