The cation‐independent mannose‐6‐phosphate receptor binds to soluble GPI‐linked proteins via mannose‐6‐phosphate

FEBS Letters

Feizi, Ten¹  Ferguson, Michael A.J.²  Robinson, Peter J.³  Green, Paula J.¹ 

[1] Glycobiology Group, The Glycosciences Laboratory, Northwick Park Hospital, Watford Road, Harrow, Middx, HA1 3UJ, UK;Carbohydrate Research Centre, Department of Biochemistry, University of Dundee, Dundee, DD1 4HN, UK;Transplantation Biology Group, Clinical Sciences Centre, RPMS, Hammersmith Hospital, Du Cane Road, London, W12 ONN, UK

关键词: Mannose-6-phosphate/insulin-like growth factor II receptor;    Glycosyl-phosphatidylinositol-linked molecule;    Phosphatidylinositol-specific phospholipase C;    Cell growth regulation;    Carbohydrate recognition;    BSA;    bovine serum albumin;    ECL;    enhanced chemiluminescence;    GPI;    glycosyl-phosphatidylinositol;    IGF-II;    insulin-like growth factor II;    MPR;    cation-independent mannose-6-phosphate/insulin-like growth factor II receptor;    mf;    membrane form of GPI-anchored protein;    PBS;    phosphate-buffered saline (10 mM phosphate buffer in 140 mM NaCl pH 74);    PI-PLC;    phosphatidylinositol-specific phospholipase C;    PPME;    phosphomannosyl ester;    s;    soluble form of GPI-anchored protein;    TBS;    Tris-buffered saline (10 mM Tris-HCl in 140 mM NaCl);    VSG;    variant surface glycoprotein;   

DOI  :  10.1016/0014-5793(95)00050-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cation-independent mannose-6-phosphate/insulin-like growth factor II receptor has been observed to bind to soluble forms of glycosyl-phosphatidylinositol-linked molecules, one of mammalian origin (rat Thy-1) and two of protozoan origins. Of the two phosphate groups found on the soluble forms of the protozoan glycosyl-phosphatidylinositol-linked molecules: (i) the internal mannose-6-phosphate diester (which forms a part of the ethanolamine bridge) and (ii) the inositol-1,2 cyclic phosphate group (which arises after cleavage of the membrane associated form with phosphatidylinositol-specific phospholipase C), only the former appears to be recognized by the mannose-6-phosphate/insulin-like growth factor II receptor, as mild acid hydrolysis which destroys the latter has been observed not to affect the receptor binding site.

【 授权许可】

Unknown