Multiple isoforms of Pisum trypsin inhibitors result from modification of two primary gene products

FEBS Letters

Welham, T.¹  Domoney, C.¹  Firmin, J.L.¹  Sidebottom, C.² 

[1] John Innes Centre, Colney Lane, Norwich, NR4 7UH, UK;Unilever Research, Colworth Laboratory, Sharnbrook, Bedford, MK44 1LQ, UK

关键词: Pisum sativum;    Gene family;    Seed development;    Trypsin inhibitor;    ESMS;    electrospray mass spectrometry;    PAGE;    polyacrylamide gel-electrophoresis;    SDS;    sodium dodecyl sulphate;    TI;    trypsin inhibitor(s);   

DOI  :  10.1016/0014-5793(95)00070-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Characterization of Pisum (pea) seed trypsin inhibitors (TI) and their corresponding cDNAs indicates that the pea TI gene family contains two genes. The existence of multiple TI isoforms can be attributed to post-translational modifications of primary gene products. Post-translational processing at the C-terminus during the desiccation stage of seed development results in the appearance of TI isoforms with increased affinity for the target enzyme, trypsin.

【 授权许可】

Unknown