Squamous cell carcinoma antigen is a potent inhibitor of cysteine proteinase cathepsin L

FEBS Letters

Takeda, Atsushi²  Nakamura, Yoshiko²  Hibino, Toshihiko¹  Yamamoto, Takako²  Takahashi, Tadahito¹ 

[1] Shiseido Research Center, 2–121 Fukuura, Kanazawa-ku, Yokohama-shi 236, Japan;Department of Clinical Pathology, Showa University Fujigaoka Hospital, Showa University School of Medicine, 1–30 Fujigaoka, Aoba-ku, Yokohama-shi 227, Japan

关键词: Squamous cell carcinoma antigen (SCCA);    Serpin;    Proteinase inhibitor;    Cathepsin L;    SCCA;    squamous cell carcinoma antigen;    FPLC;    fast protein liquid chromatography;    HPLC;    high performance liquid chromatography;    Boc;    t-butyloxycarbonyl;    Suc;    succinyl;    Z;    carbobenzoxy;    Suc(OMe);    N-methoxysuccinyl;    MCA;    4-methylcoumaryl-7-amide;    DTT;    dithiothreitol;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(94)01456-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A squamous cell carcinoma antigen (SSCA), which is a member of the serpin family of proteinase inhibitors, was purified from sera of cancer patients. It did not inhibit serine proteinases. However, it non-competitively inhibited human cathepsin L with a K _i of 0.064 nM, but not cathepsins B and H among cysteine proteinases. These results indicated that SCCA is a non-functional serpin that inhibits cathepsin L in cancer cells.

【 授权许可】

Unknown