Prolactin induces growth inhibition and promotes differentiation of CHO cells stably transfected with prolactin receptor complementary DNA

FEBS Letters

Djiane, Jean¹  Bignon, Christophe¹  Daniel, Nathalie¹  Kermabon, Anne-Yvonne¹ 

[1] Unité d'Endocrinologie Moléculaire, Institut National de la Recherche Agronomique, 78352 Jouy-en-Josas Cédex, France

关键词: Prolactin receptor;    Growth inhibition;    Differentiation marker;    Signal transduction;    Transfection;    oPRL;    ovine prolactin;    kb;    kilobases;    CAT;    chloramphenicol acetyl transferase;    mAb;    monoclonal antibody;    pAb;    polyclonal antibody;    Ig;    immunoglobulins;    GH-R cDNA;    growth hormone receptor complementary DNA;    LIF;    leukemia inhibitory factor;    CNTF;    cilliary neurotrophic factor;    OM;    oncostatin M;    EPO;    erythropoietin;    rbPRL-R;    rabbit prolactin receptor;   

DOI  :  10.1016/0014-5793(94)01400-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have characterized a stable and functional transfectant of the rabbit prolactin receptor in Chinese hamster ovary cells, and investigated the action of prolactin (PRL) on the growth and differentiation of this transfectant (clone E32). PRL induced a significant inhibition of E32 cell proliferation. Growth inhibition correlated with gene induction of the molecular marker of ovarian differentiation cholesterol side chain cleavage P450 (P450_scc). Both effects were inversely proportional to cell confluence. The limits and potential development of such transfected cellular systems are discussed.

【 授权许可】

Unknown