Stoichiometry of the EF‐Tu · GTP complex with aminoacyl‐tRNA: ternary of quinternary?

FEBS Letters

Leberman, Reuben¹ 

[1] European Molecular Biology Laboratory, Grenoble Outstation, BP 156, 38042 Grenoble Cedex, France

关键词: Elongation factor Tu;    Aminoacyl-tRNA;    Deacylation;    Ternary complex;    Quinternary complex;   

DOI  :  10.1016/0014-5793(94)01398-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The stoichiometry of the complex formed between the Escherichia coli polypeptide elongation factor EF-Tu, GTP and valyl-tRNA^val has been determined by non-enzymatic deacylation studies on mixtures of the components at well-defined concentrations. A titration end-point was found corresponding to a 1:1 complex of EF-Tu · GTP with the aminoacylated-tRNA i.e. formation of a ternary complex. The result conforms to the classical model of the elongation step and not to the revolutionary proposition of the formation of a 2:2:1 complex; quinternary complex (EF-Tu · GTP)₂ · as-RNA.

【 授权许可】

Unknown